In summary, this protocol shows that expression of the NahG transgene greatly enhances the efficiency of Agrobacterium-mediated transformation in rosette leaves in Arabidopsis, enabling the routine use of this technique in the model plant. You are interested in live and on-demand webinars and demonstrations Eppendorf Lab Channel is a virtual platform where anyone can watch webinars for free. Remarkably, this protocol for transient expression can be used with, at least, three widely used Agrobacterium strains, LBA4404, GV3101 and C58C1. ), we have been able to obtain high accumulation of marker proteins such as GUS and GFP, and carry out sub-cellular localization and protein-protein interaction experiments. Using Arabidopsis NahG plants, which contain low levels of salicylic acid (SA) due to the expression of an SA hydroxylase from the bacterium Pseudomonas putida ( Eppendorf offers a choice of preventive maintenance services and service agreement options for consistent. Recently, we described a protocol to perform transient gene expression using NahG Arabidopsis plants, overcoming previous limitations (Rosas. Many efforts have been made in the past to increase the efficacy of Agrobacterium-mediated transient transformation in Arabidopsis (reviewed in benthamiana as a heterologous system, which entails obvious limitations and might generate misleading results. On the other hand, these assays are inefficient and lack robustness when carried out in the model plant Arabidopsis thaliana (hereafter referred to as Arabidopsis), forcing Arabidopsis researchers to use N. These methods have many advantages over the laborious and time-consuming stable transformation approaches including, among others, a higher efficiency, simplicity, and fast, consistent results when the transient transformation assays are carried out in Nicotiana benthamiana. The Eppendorf Research plus microlitre pipette has been developed within the strict criteria of the Eppendorf PhysioCare Concept® which ensures an ultralight and balanced pipette equipped with the unique spring-loaded tip cone and SOFTeject function.Agrobacterium tumefaciens (hereafter referred to as Agrobacterium)-mediated transient transformation assays have greatly contributed to research in molecular plant biology. The less strain you experience, the more energy you have for your experiments. All Eppendorf microlitre pipettes up to 1,000 µL are equipped with this spring-loaded tip cone.įeel the difference in weight, pipetting forces and the spring loaded tip cone. The CellXpert ® CO 2 incubator family are the first cell culture incubators engineered and produced by Eppendorf in Hamburg, Germany. If present, remove the four lifting handles. Cell culture incubator CellXpert ® Culture of Tomorrow Todays cell culture requires more than technical solutions for optimized growth conditions and efficient contamination prevention. It provides increased user-to-user reproducibility for more uniform results among members of the lab. Position the upper incubator so that the doors of the stacked devices are flush. The spring limits the attachment forces and at the same time guarantees a reproducible and safe tip fit. Just one gentle push-down until the haptic feedback indicates a perfect and reliable tip fit - no need for rocking and banging. The spring-loaded tip cone saves you energy and time. Spring-loaded tip cone for reliable tip fit and low tip attachment forces Promo bundle consists of: 1x S41i CO 2 incubator shaker, 170 L, with orbit 2.5 cm (1 in) and 1x universal platform. Eppendorf Reference 2 Adjustable Volume, Single Channel Pipette, 20 - 200 uL. Enjoy excellent flexibility and choose among single-channel pipettes in fixed or variable volume as well as multi-channel pipette options with 8, 12, 16 and 24 channels. for versatile mammal cell culture applications such as protein expression or cell line establishment. Incubator Dry Bath Hotplates Vacuum Pumps Fluid. Research plus pipettes have the lowest weight and lowest operation forces in the Eppendorf product families. 1 ASSAY SPAchip eppendorf tube 1 Control.

The Research plus microlitre pipette protects the health of our customers during their daily work in the laboratory with reduced hand and arm strain during pipetting. Incubator CERO 3D 3D Cell Culture CEROplates, ultra-low attachment. Get the Eppendorf standard in deep well plate format our high-performance plates come in 96- and 384-well format with OptiTrack matrix for the highest. It provides you with one of the safest and most ergonomic pipettes available today. The Eppendorf Research plus mechanical pipette combines about 60 years of innovation in liquid handling and is one of the most commonly used pipettes in the world.

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